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The levels of oxidative stress were detected by CellROX Deep Red staining (Fig
3a), suggesting that feminized mutants displayed a gene expression profile that is more similar to fully reproductive adults than to germline-less or masculinized mutants
Beads were pelleted, washed, PNGase F-treated (G5166, Sigma) (if applicable), and proteins eluted in SDS sample buffer/2.5% mercaptoethanol
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